Journal: Nature Communications

Article Title: Circulating myocardial microRNAs from infarcted hearts are carried in exosomes and mobilise bone marrow progenitor cells

doi: 10.1038/s41467-019-08895-7

Figure Lengend Snippet: Myo-miRs are released into PB following AMI and transported into BM-MNCs. AMI and Sham surgeries were performed in C57BL/6 mice; then at various time points, the plasma, BM-MNCs, and different organs were isolated and subjected to qRT-PCR analyzes of myo-miRs, miR-1a, 208a, 133a, and 499-5p. a myo-miR levels in the plasma 6 h post-surgery ( n = 10 animals per group). b myo-miR levels in the BM-MNCs, kidney, spleen, and liver 12 h post-surgeries, expressed relative to the levels in the intact controls ( n = 5 animals per group), and c the fold difference in AMI vs. Sham mice ( n = 5 animals per group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Sham; n.s., no significant. d The fold difference of myo-miR levels in the BM-MNCs of AMI vs. Sham mice at 0, 6, 12, 24, 72, and 120 h post-surgery. * p < 0.05 ** p < 0.01, *** p < 0.001 vs. Sham at same time point. n = 5 animals per group per time point. An unpaired t test was used in a and a two-way ANOVA was used in b , c , and d for statistical analysis. Error bars represent mean ± s.e.m

Article Snippet: MicrOFFTM myo-miRs (miR-1a, miR-208a, miR-133a and miR-499-5p) antagomirs and micrOFFTM miRNA antagomir Negative Control were synthesized by RiboBio Co., Ltd.

Techniques: Clinical Proteomics, Isolation, Quantitative RT-PCR

Journal: Nature Communications

Article Title: Circulating myocardial microRNAs from infarcted hearts are carried in exosomes and mobilise bone marrow progenitor cells

doi: 10.1038/s41467-019-08895-7

Figure Lengend Snippet: Myo-miRs downregulate CXCR4 expression in BM-MNCs and MSCs in vitro. BM-MNCs (2 × 10 6 /well) and MSCs (0.5 × 10 6 /well) were transfected with synthesized miR-1a, miR-208a, miR-133a, or miR-499-5p mimics or with a scrambled miR (final concentrations: 50 nM); 24 h later, CXCR4 mRNA expression in the BM-MNCs ( a , c , e ) and MSC ( b , d , f ) was quantified by RT-PCR ( a , b ), Western-blotting ( c , d ), and flow cytometry ( e , f ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Scrambled. n = 5 biologically independent samples per group. A one-way ANOVA was used for statistical analysis. Error bars represent mean ± s.e.m

Article Snippet: MicrOFFTM myo-miRs (miR-1a, miR-208a, miR-133a and miR-499-5p) antagomirs and micrOFFTM miRNA antagomir Negative Control were synthesized by RiboBio Co., Ltd.

Techniques: Expressing, In Vitro, Transfection, Synthesized, Reverse Transcription Polymerase Chain Reaction, Western Blot, Flow Cytometry

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Effect of miR-18a overexpression on the radiosensitivity of non-small cell lung cancer

doi:

Figure Lengend Snippet: Clinicopathological variables and the expression of miR-18a in total NSCLC patients

Article Snippet: For the transfection, 1 × 10 6 cells were treated with 50 nM GMR-miRTM inhibitor miR-18a/inhibitor NC (Shanghai GenePharma) in 1 μl of Lipofectamine TM 2000 (Invitrogen, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Effect of miR-18a overexpression on the radiosensitivity of non-small cell lung cancer

doi:

Figure Lengend Snippet: The expression of miR-18a in lung cancer tissues and matched normal tissues.

Article Snippet: For the transfection, 1 × 10 6 cells were treated with 50 nM GMR-miRTM inhibitor miR-18a/inhibitor NC (Shanghai GenePharma) in 1 μl of Lipofectamine TM 2000 (Invitrogen, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Effect of miR-18a overexpression on the radiosensitivity of non-small cell lung cancer

doi:

Figure Lengend Snippet: The expression of miR-18a in A549 lung cancer cell line, and NHBE cell line.

Article Snippet: For the transfection, 1 × 10 6 cells were treated with 50 nM GMR-miRTM inhibitor miR-18a/inhibitor NC (Shanghai GenePharma) in 1 μl of Lipofectamine TM 2000 (Invitrogen, USA) according to the manufacturer’s instructions.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Effect of miR-18a overexpression on the radiosensitivity of non-small cell lung cancer

doi:

Figure Lengend Snippet: Correlation of miR-18a level and response to radiotherapy

Article Snippet: For the transfection, 1 × 10 6 cells were treated with 50 nM GMR-miRTM inhibitor miR-18a/inhibitor NC (Shanghai GenePharma) in 1 μl of Lipofectamine TM 2000 (Invitrogen, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Biomarker Discovery

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Effect of miR-18a overexpression on the radiosensitivity of non-small cell lung cancer

doi:

Figure Lengend Snippet: MiR-18a-regulating sensitivity of A549 cells to radiation. The A549 cells transfected with inhibitor miR-18a or inhibitor NC were treated with 0, 2, 4, 6, 8, and 10 Gy irradiation, and survival curves were determined using a colony-forming assay. *P < 0.05.

Article Snippet: For the transfection, 1 × 10 6 cells were treated with 50 nM GMR-miRTM inhibitor miR-18a/inhibitor NC (Shanghai GenePharma) in 1 μl of Lipofectamine TM 2000 (Invitrogen, USA) according to the manufacturer’s instructions.

Techniques: Transfection, Irradiation